Distinct inflammatory profiles distinguish COVID-19 from influenza with limited contributions from cytokine storm

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paper: Mudd, P. A., Crawford, J. C., ..., Powderly, W. G., Thomas, P. G. and Ellebedy, A. H. (2020). Distinct inflammatory profiles distinguish COVID-19 from influenza with limited contributions from cytokine storm. Sci Adv https://www.ncbi.nlm.nih.gov/pubmed/33187979/
contributor: Philip A. Mudd
contributor_organization: Washington University School of Medicine
contributor_email: pmudd@wustl.edu

 

    • description: The dataset in our manuscript includes: 1) 35-plex plasma cytokine concentration measurements from 168 individuals with COVID-19, 26 individuals with seasonal influenza and 16 healthy controls; 2) multiparameter flow cytometry from 22 COVID-19, 23 seasonal influenza and 15 healthy controls; 3) single-cell RNA transcriptional profiles from 3 individuals with severe COVID-19, 3 individuals with severe seasonal influenza and 2 healthy controls.
    • exact_source: Multiple signatures are highlighted in several figures. The cytokine/FACS data are found in Supplementary Material Table S1. Single-cell RNAseq raw data is found in the repositories listed below.
    • tissue: plasma and PBMC
    • immune_exposure: SARS-CoV-2 infection or seasonal influenza infection of varying clinical severity or uninfected controls
    • cohort: adults age 18-92
    • comparison: influenza vs. COVID-19 vs. healthy control; single-cell RNAseq is severe influenza vs. severe COVID-19
    • repository_id: Single-cell gene expression data have been uploaded to the National Center for Biotechnology Information (NCBI) Short Read Archive under BioProject ID PRJNA630932 and SRA accession SRR11233662.
    • platform: Cytokine measurement - Luminex FLEXMAP 3D; Single-cell RNAseq - 10x Genomics 5' (V2) kit with sequencing on Illumina NovaSeq 6000.
    • response_components: multiple responses delineated in manuscript - largest unifying difference is lower type I and type II interferon signaling in COVID-19 when compared with influenza
    • response_behavior: multiple response behaviors delineated in manuscript

 

PMID
33187979
authors
Mudd, Philip A. et al
abstract
We pursued a study of immune responses in coronavirus disease 2019 (COVID-19) and influenza patients. Compared to patients with influenza, patients with COVID-19 exhibited largely equivalent lymphocyte counts, fewer monocytes, and lower surface human leukocyte antigen (HLA)–class II expression on selected monocyte populations. Furthermore, decreased HLA-DR on intermediate monocytes predicted severe COVID-19 disease. In contrast to prevailing assumptions, very few (7 of 168) patients with COVID-19 exhibited cytokine profiles indicative of cytokine storm syndrome. After controlling for multiple factors including age and sample time point, patients with COVID-19 exhibited lower cytokine levels than patients with influenza. Up-regulation of IL-6, G-CSF, IL-1RA, and MCP1 predicted death in patients with COVID-19 but were not statistically higher than patients with influenza. Single-cell transcriptional profiling revealed profound suppression of interferon signaling among patients with COVID-19. When considered across the spectrum of peripheral immune profiles, patients with COVID-19 are less inflamed than patients with influenza.
status
review complete
curator
Hancock Lab
reviewer
Aris Floratos
journal
Sci Adv
date review completed
edit spreadsheet url
https://docs.google.com/spreadsheets/d/1YgRtOf_dqFO-gl7E3NKIm3wmE2kqVkg__ZGgVJr…
view spreadsheet url
https://docs.google.com/spreadsheets/d/1YgRtOf_dqFO-gl7E3NKIm3wmE2kqVkg__ZGgVJr…
manuscript url
https://www.ncbi.nlm.nih.gov/pubmed/33187979/
year of publication
2020
In Dashboard
Yes