Manuscript Detail

Authors: Law, J. C. et al
Abstract: There is a pressing need for an in-depth understanding of immunity to SARS-CoV-2. Here we investigated T cell recall responses to fully glycosylated Spike trimer, recombinant N protein as well as to S, N, M and E peptide pools in the early convalescent phase. All subjects showed SARS-CoV-2-specific T cell responses to at least one antigen. SARS-CoV-2-specific CD4+ T cells were primarily of the central memory phenotype and exhibited a lower IFN-[gamma] to TNF-[alpha] ratio compared to influenza-specific responses of the same donors, independent of disease severity. SARS-CoV-2-specific T cells were less multifunctional than influenza-specific T cells, particularly in severe cases, potentially suggesting exhaustion. High IL-10 production was noted in response to N protein, possibly contributing to immunosuppression, with potential implications for vaccine design. We observed granzyme B+/IFN-[gamma] CD4+ and CD8+ proliferative responses to peptide pools in most individuals, with CD4+ responses predominating over CD8+ responses. Peripheral T follicular helper responses to S or N strongly correlated with serum neutralization assays as well as RBD-specific IgA. Overall, T cell responses to SARS-CoV-2 are robust, however, CD4+ Th1 responses predominate over CD8+ responses and are more inflammatory with a weaker Tfh response than influenza-specific CD4+ responses, potentially contributing to COVID-19 disease.
Curator: Hancock Lab
Reviewer: Aris Floratos
Author-provided data: ?

paper: Law, J. C., Koh, W. H., Budylowski, P., Lin, J., Yue, F., Abe, K. T., ... & Ostrowski, M. A. (2020). Systematic Examination of Antigen-Specific Recall T Cell Responses to SARS-CoV-2 versus Influenza Virus Reveals a Distinct Inflammatory Profile. The Journal of Immunology, 206(1), 37-50.
contributor: Tania H. Watts
contributor_organization: University of Toronto


    • description: Analysis of T cell responses to SARS-CoV-2 using multiparameter flow cytometry, Multiplex cytokine assays and proliferation assays on 13 leukapheresis samples from subjects in early convalescent phase of COVID19, range of disease severity from asymptomatic to ICU.
    • exact_source:
    • tissue: PBMC
    • immune_exposure: PCR Confirmed COVID19 27-90d post symptoms
    • cohort: Adults (range 31-72, average 53 y.o.)
    • comparison: 13 subjects,range severe to asymptomatic
    • repository_id:
    • platform:
    • response_components:
    • response_behavior: