Manuscript Detail

Authors: Wang, Jun et al
Abstract: Background: Cases of excessive neutrophil counts in the blood in severe coronavirus disease (COVID-19) patients have drawn significant attention. Neutrophil infiltration was also noted on the pathological findings from autopsies. It is urgent to clarify the pathogenesis of neutrophils leading to severe pneumonia in COVID-19. Methods: A retrospective analysis was performed on 55 COVID-19 patients classified as mild (n = 22), moderate (n = 25), and severe (n = 8) according to the Guidelines released by the National Health Commission of China. Trends relating leukocyte counts and lungs examined by chest CT scan were quantified by Bayesian inference. Transcriptional signatures of host immune cells of four COVID19 patients were analyzed by RNA sequencing of lung specimens and BALF. Results: Neutrophilia occurred in 6 of 8 severe patients at 7–19 days after symptom onset, coinciding with lesion progression. Increasing neutrophil counts paralleled lesion CT values (slope: 0.8 and 0.3–1.2), reflecting neutrophilia-induced lung injury in severe patients. Transcriptome analysis revealed that neutrophil activation was correlated with 17 neutrophil extracellular trap (NET)-associated genes in COVID-19 patients, which was related to innate immunity and interacted with T/NK/B cells, as supported by a protein–protein interaction network analysis. Conclusion: Excessive neutrophils and associated NETs could explain the pathogenesis of lung injury in COVID-19 pneumonia.
Journal: Front Immunol
Curator: Hancock Lab
Reviewer: Aris Floratos
Author-provided data: ?

paper: Wang, J., Li, Q., Yin, Y., Zhang, Y., Cao, Y., Lin, X., Huang, L., Hoffmann, D., Lu, M. and Qiu, Y. (2020). Excessive Neutrophils and Neutrophil Extracellular Traps in COVID-19. Front Immunol
contributor: Daniel Hoffmann, Mengji Lu, Yuanwang Qiu
contributor_organization: University of Duisburg-Essen and Jiangnan University


    • description: Transcriptome analysis of the lung and BALF in COVID-19 patient. Marker genes were used to identify Neutrophils, T cells, Monocytes, and B cells in both Lung and BALF samples of COVID-19 patients and healthy controls, respectively.
    • exact_source: Figure 4
    • tissue: Lung and BALF
    • immune_exposure: COVID-19 infection
    • cohort: Old Adult (>60 years) for GSE147507, Adult (18-60 years) for CRA002390
    • comparison: COVID-19 patients and healthy controls
    • repository_id: CRA002390, GSE147507
    • platform: Illumina NextSeq 500
    • response_components: LGALS9, HCK, LCP1, CEACAM1, S100A8, LGALS9, CTSC
    • response_behavior: up